Transforming Growth Factor β (TGF-β)Inhibits Expression of Fibrinogen and Factor VII in a Hepatoma Cell Line

Jane H Hassan; Cristiana Chelucci; Cesare Peschle; Vincenzo Sorrentino

doi:10.1055/s-0038-1648474

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1992; 67(04): 478-483
DOI: 10.1055/s-0038-1648474

Original Articles

Schattauer GmbH Stuttgart

Transforming Growth Factor β (TGF-β)Inhibits Expression of Fibrinogen and Factor VII in a Hepatoma Cell Line

Jane H Hassan

¹The Department of Hematology and Oncology, Istituto Superiore di Sanita, Rome, Italy

²European Molecular Biology Laboratory, Heidelberg, FRG

,

Cristiana Chelucci

¹The Department of Hematology and Oncology, Istituto Superiore di Sanita, Rome, Italy

,

Cesare Peschle

¹The Department of Hematology and Oncology, Istituto Superiore di Sanita, Rome, Italy

,

Vincenzo Sorrentino

²European Molecular Biology Laboratory, Heidelberg, FRG

› Author Affiliations

Abstract

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Summary

We have investigated the effect(s) of transforming growth factor (TGF)-β1 and interleukin (IL)-6 on the expression of fibrinogen and blood coagulation factors VII, IX, X mRNAs in a hepatoma cell line (Hep 3B). The results indicate that TGF-β induces a decrease of the basal level of fibrinogen and factor VII mRNAs, but does not affect factor X expression. Furthermore, TGF-β efficiently antagonizes the IL-6 induction of fibrinogen mRNA at late (12-48 h) but not early (6 h) times: this effect is apparently mediated by posttranscriptional mechanism(s). These findings, together with previously reported data on the inhibitory effect of TGF-β on acute phase genes (e.g., ApoAl and albumin), suggest a role for TGF-β in the regulation of liver genes expression. The early stimulatory and late inhibitory effect exerted by IL-6 and TGF-β respectively on fibrinogen mRNA level may play a role in the regulatory mechanism(s) of clot formation in a variety of conditions.

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References

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